Journal: bioRxiv

Article Title: Exploiting macropinocytosis for therapeutic intervention in RAS mutant Multiple Myeloma

doi: 10.1101/2025.04.04.647098

Figure Lengend Snippet: A) Schematic of the monobody protein and drug conjugate with MMAE. B) Fluorescent images (left) and quantification (right) of FN-Cy5.5 in MM cell lines L363 and RMPI 8226 treated with vehicle or EIPA. C) Mutant RAS and WT RAS cell lines from multiple cancer types were treated with MMAE and FN-MMAE. Cytotoxic ratio (FN-MMAE IC 50 / MMAE IC 50 ) values were plotted to normalize differences in chemotherapeutic sensitivity between cell lines. D) IC 50 s of SoC chemotherapy (Pomalidomide, Bortezomib, and Doxorubicin) and FN-MMAE in KMS11 versus KMS11 KRASV 12 cells. E) Schematic of the treatment regimen used in F-H is shown. F-H) Tumors in mice bearing L363 (F), KMS11 KRAS V12 (G), or KMS11 (H) were measured twice weekly to plot growth curves. All images are representative. Scale bar for all images, 10μm. For FN-Cy5.5 assays, at least 500 cells were counted per biological replicate (n=3). *, P < 0.05; **, P < 0.005; ***, P < 0.0005; ****, P < 0.00005, as determined by an unpaired, two-tailed Student’s t-test. For B and C, error bars indicate mean ± SD of biological replicates (n=3). For F-H, error bars indicate mean ± SEM (n=8-10).

Article Snippet: Fluorescent probes sulfo-cyanine cy5.5 carboxylic acid (17390) and cyanine5.5 (Cy5.5) maleimide (27080) were from Lumiprobe.

Techniques: Mutagenesis, Two Tailed Test

Journal: Advanced Science

Article Title: Whole‐Tumor Clearing and Imaging of Intratumor Microbiota in Three Dimensions with miCDaL Strategy

doi: 10.1002/advs.202400694

Figure Lengend Snippet: 3D views of spatial distribution between intratumor microbiota and activated T ‐cells. a) Schematic diagram showing the workflow of 3D imaging of native intratumor microbiota and related tumor immune microenvironment by the integrated antibody immunostaining. b) 3D view of a whole tumor from MMTV‐PyMT mice. Activated T ‐cells are indicated by CD4 and CD8 antibodies. Intratumorally injected TADA was used for the in vivo labeling of intratumor microbes. Blue: CD4, Yellow: TADA, Red: CD8, White: DAPI. Scale bar, 2000 µm. Representative results from three independent experiments are shown.

Article Snippet: Two fluorescent D‐amino acids (FDAAs) probes, TAMRA‐amino‐D‐alanine (TADA) and Cy5‐amino‐D‐alanine (Cy5ADA), were purchased from Shanghai Biochempartner Co., Ltd. (Shanghai, China).

Techniques: Imaging, Immunostaining, Injection, In Vivo, Labeling